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Updated: Jul 6, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
The human milk endocannabinoidome is preserved by high hydrostatic pressure processing but altered by holder
Lucie Marousez1, Elizabeth Dumais2,3, Léa Chantal Tran4
1Univ. Lille, Inserm, CHU Lille, U1286-INFINITE-Institute for Translational Research in Inflammation, Lille, France.
Introduction:
The microbiological safety of donor milk (DM) is commonly ensured by holder pasteurization (HoP, 62.5°C for 30 min) in human milk banks despite its detrimental effects on several bioactive factors. We compared the concentration of twelve endocannabinoid (eCB)-like mediators in raw DM and in DM after holder pasteurization or high hydrostatic pressure processing (HHP, 350 MPa at 38°C), a non-thermal substitute for DM pasteurization.
Methods:
We measured five N-acyl-ethanolamines (NAEs) and seven 2-mono-acyl-glycerols (2-MAGs) in raw-DM, HHP-DM and HoP-DM using high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
Results:
HoP-DM and HHP-DM demonstrated comparable concentrations compared with raw DM of 2-MAGs as well as for N-docosapentaenoyl-ethanolamine (DHEA, an NAE). However, four other NAEs, including N-arachidonoyl-ethanolamine (AEA), N-palmitoyl-(PEA), N-oleoyl -ethanolamine (OEA) and N-linoleoyl-ethanolamine (LEA) were significantly increased by HoP.
Conclusion:
Our study suggests that HHP-DM may more suitable than HoP-DM to improve the development of preterm infants through the preservation of milk eCB mediators at level close to their initial levels in raw DM.
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