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Reversing Oxinflammation Associated with Glycative Stress and Formation of Advanced Glycation End Products with a
Anna Guiotto1, Alessandra Pecorelli1, Zoe Diana Draelos2
1Drs. Guiotto and Pecorelli are with the Department of Environmental and Prevention Sciences at the University of Ferrara in Ferrara, Italy.
Objective:
Skin aging is accelerated by glycative stress, which promotes the accumulation of advanced glycation end products (AGEs) and impairs the extracellular matrix. A randomized, double-blinded, placebo-controlled trial evaluated a dietary supplement containing rosemary extract (BioR), demonstrating tissular and visible improvements in skin quality. The data reported herein evaluated markers associated with glycative stress and AGEs from skin biopsies and tape strips obtained following dietary supplement use.
Methods:
Female participants (N=104), aged 40 to 65 years, with moderate-to-severe skin dullness and roughness/texture, and mild-to-moderate erythema, pore size, and uneven pigmentation were randomized to BioR (n=52) or placebo ([PLB] n=52). Capsules were taken with food over 12 weeks. Subjects (n=16, BioR; n=16, PLB) underwent 3mm punch biopsies (volar upper arm) and tape stripping (16 tape strips, each; volar forearm) at baseline and 12 weeks for analysis of 4-hydroxynonenal protein adducts (4HNE [oxidative stress marker]) and AGEs.
Results:
Immunohistochemistry and ELISA revealed that levels of 4HNE protein adducts were significantly decreased from baseline in the BioR versus PLB group (p<0.005; biopsies) and significantly decreased from baseline in the BioR group alone (p<0.05; tape strips) at 12 weeks. Significant reductions in AGEs occurred in the BioR versus PLB group (p<0.005; biopsies) at 12 weeks. No significant changes from baseline occurred in 4HNE protein adduct levels or AGEs in the PLB group.
Conclusion:
After 12 weeks, a dietary supplement containing rosemary extract led to significant reductions in a marker associated with oxidative stress, a component of glycation, and AGEs versus placebo in skin in addition to visible improvements in skin quality.
Insights
A rosemary extract supplement significantly reduced skin aging markers, including advanced glycation end products (AGEs) and oxidative stress, compared to placebo. This dietary intervention improved skin quality and reduced visible signs of aging over 12 weeks.
Area of Science:
- Dermatology and Cosmetology
- Biochemistry of Aging
- Nutraceutical Research
Background:
- Skin aging is exacerbated by glycative stress, leading to advanced glycation end products (AGEs) accumulation.
- Glycation and oxidative stress degrade the skin's extracellular matrix, impairing skin quality.
- Dietary interventions show potential for mitigating skin aging processes.
Purpose of the Study:
- To evaluate the efficacy of a rosemary extract dietary supplement (BioR) in reducing markers of glycative and oxidative stress in the skin.
- To assess the impact of BioR on advanced glycation end products (AGEs) and 4-hydroxynonenal protein adducts (4HNE).
- To correlate biochemical improvements with visible changes in skin quality.
Main Methods:
- A 12-week, randomized, double-blind, placebo-controlled trial involving 104 female participants aged 40-65.
- Participants received either BioR or placebo capsules daily.
- Skin biopsies and tape strips were analyzed for AGEs and 4HNE protein adducts at baseline and 12 weeks.
Main Results:
- BioR significantly reduced 4HNE protein adducts in skin biopsies and tape strips compared to placebo.
- Significant reductions in AGEs were observed in the BioR group versus placebo in skin biopsies.
- No significant changes in these markers were observed in the placebo group.
Conclusions:
- A 12-week dietary supplement with rosemary extract effectively reduced skin AGEs and oxidative stress markers.
- The supplement demonstrated significant improvements in skin quality, correlating with reduced biochemical markers of aging.
- Rosemary extract shows promise as a nutraceutical intervention for combating skin aging.
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