Related Experiment Video
Updated: May 2, 2026
08:27
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Published on: March 24, 2015
13.8K
Dual-Functionalized Glass Micropipette Sensor for Simultaneous High Sensitivity Detection of Cancer Biomarkers
Guofeng Wang1, Shiwei Xu1, Yueyue Feng1
1Key Lab of Biohealth Materials and Chemistry of Wenzhou, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325027, Zhejiang, P. R. China.
ACS Applied Materials & Interfaces
|March 26, 2025
Summary
This study introduces a novel dual-functionalized sensor for early cancer detection. It simultaneously identifies nucleic acids and proteins, improving accuracy and survival rates.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cancer Diagnostics
Background:
- Early cancer detection significantly improves patient survival.
- Current single-biomarker methods lack sensitivity and accuracy, leading to false positives.
- There is a need for advanced diagnostic tools capable of detecting multiple cancer biomarkers simultaneously.
Purpose of the Study:
- To develop and validate a dual-functionalized glass micropipet sensor (DFMS) for simultaneous detection of cancer biomarkers.
- To enhance sensitivity and selectivity in cancer detection by combining ionic current and Raman-based methods.
- To provide a novel approach for early cancer detection with potential clinical implications.
Main Methods:
- Fabrication of a dual-functionalized glass micropipet sensor (DFMS).
- Inner surface functionalization with amino-modified silicon nanowires (SiNWs) for miRNA capture and ionic-current detection.
- Outer surface decoration with gold nanoparticles for protein aptamer anchoring and Raman-based detection.
- Simultaneous detection of nucleic acids (miRNAs) and proteins.
Main Results:
- Achieved detection limits of 1 aM for miRNAs and 0.001 ng/mL for proteins.
- Demonstrated minimal mutual interference between ionic current and Raman detection modes.
- Validated sensor performance using prostate and gastric cancer biomarkers (miRNA-1246, PSA, miRNA-106a, CD44).
- Exhibited outstanding sensitivity, selectivity, stability, and broad applicability.
Conclusions:
- The DFMS offers a highly sensitive and selective platform for simultaneous detection of multiple cancer biomarkers.
- This dual-modal sensing approach overcomes limitations of single-biomarker detection methods.
- The developed sensor shows significant potential for early cancer diagnosis and improved patient outcomes.
