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Chronic Oral D-Galactose Induces Oxidative Stress but Not Overt Organ Dysfunction in Male Wistar Rats
Jelena Martinovic1, Ivana Gusevac Stojanovic1, Sladjan Nesic2
1Department of Molecular Biology and Endocrinology, VINCA Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.
Current Issues in Molecular Biology
|March 26, 2025
Summary
Oral administration of D-galactose (d-gal) in rats induced oxidative stress, mimicking natural aging effects. The liver showed the most damage, but organs largely maintained function, suggesting a milder aging model compared to injections.
Area of Science:
- Biochemistry
- Gerontology
- Toxicology
Background:
- D-galactose (d-gal) is an energy source and glycoconjugate component.
- Excessive d-gal can promote oxidative stress and is used in aging studies via injections.
- Oral administration of d-gal has not been extensively studied for aging effects.
Purpose of the Study:
- To investigate the systemic effects of chronic oral D-galactose administration.
- To analyze oxidative stress, histopathology, and serum biochemistry in rat organs.
- To compare oral d-gal effects with natural aging and other administration routes.
Main Methods:
- Rats were orally administered d-gal (200 mg/kg and 500 mg/kg) for 6 weeks.
- Oxidative stress markers, histopathology, and serum biochemistry were analyzed in liver, kidney, and heart.
- Effects were compared across different rat ages (12-30 months) and administration routes.
Main Results:
- Chronic oral d-gal administration induced oxidative stress in rat organs.
- The liver exhibited the most significant oxidative damage.
- Oral d-gal mimicked some aging aspects seen in 30-month-old rats, with minor histopathological changes and unaffected organ function.
Conclusions:
- Oral D-galactose administration is a viable model for inducing oxidative stress and mimicking aspects of natural aging.
- This method appears to cause milder effects than intraperitoneal or subcutaneous injections.
- The oral d-gal model shows potential for aging research without causing severe organ dysfunction.
