Detection of Exosomal microRNA from Malignant Lung Cells Using a Surface Plasmon Resonance Imaging Biosensor

The consideration of micro ribonucleic acid (microRNA) molecules is justified by their ability to act as possible biomarkers, which may be used to detect the most prevalent type of lung cancer: non-small-cell lung cancer (NSCLC). This article describes a surface plasmon resonance imaging (SPRi) biosensor, which was considered to detect malignant lung cells using an Au/Ag heterostructure, as well as the DNA tetrahedron framework (DNATF). Considering the Au/Ag heterostructure and the DNATF, the proposed SPRi biosensor was evaluated. Thus, its detection range belongs to the interval 1.82 fM up to 28 nM. The measured limit of detection of this biosensor is 1.55 fM, and the generated images offer sufficient fidelity to conduct a precise medical analysis. Therefore, patients who suffer from NSCLC may be accurately and efficiently determined. The experimental evaluation, which is presented, suggests that this is a biosensor that is capable of optimizing the clinical detection of NSCLC.