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A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in Morchella
Weilin Feng1, Zier Guo1,2, Qunli Jin1
1Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Abstract:
Based on transcriptome and proteome sequencing technologies, this study aims to preliminarily reveal the molecular mechanisms of growth and development and related metabolic regulation in Morchella sextelat. A total of 42.31 GB of Clean Data was acquired from the transcriptome sequencing (RNA-seq) of six samples in two development phases (n = 3) of M. sextelata. In the young phase (YP) and harvest phase (HP), there were 2887 differentially expressed genes (DEGs), including 1910 up-regulated genes and 977 down-regulated genes. In YP and HP, there were 987 differentially expressed proteins (DEPs), including 417 up-regulated ones and 570 down-regulated ones. Based on GO and KEGG analysis, significant differences in the transcriptomes and proteins in metabolic pathways are disclosed. Glycometabolism, especially starch, saccharose, and polysaccharide metabolism, plays a crucial role in the growth of M. sextelata. In addition, expression changes in the genes related to selenium metabolism are here recognized. These research results not only offer strong support for further exploration of the biological significance and functional differences of M. sextelata, but are also conducive to discovering key genes and understanding their regulation network during growth.
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