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Advancing Dermatomycosis Diagnosis: Evaluating a Microarray-Based Platform for Rapid and Accurate Fungal Detection-A
Vittorio Ivagnes1, Elena De Carolis2, Carlotta Magrì1
1Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Largo A. Gemelli 8, 00168 Rome, Italy.
Journal of Fungi (Basel, Switzerland)
|March 26, 2025
Summary
This study shows the EUROArray Dermatomycosis Platform accurately identifies fungi causing skin and nail infections. This molecular assay offers faster results and detects more cases than traditional methods.
Area of Science:
- Medical Mycology
- Molecular Diagnostics
Background:
- Dermatomycosis, particularly onychomycosis, causes chronic issues and recurrence, impacting quality of life.
- Conventional diagnostic methods for dermatomycosis often lack sensitivity, specificity, and speed.
Purpose of the Study:
- To evaluate the EUROArray Dermatomycosis Platform, a microarray assay, for identifying fungal pathogens in dermatomycosis.
- To compare the molecular assay's performance against microscopy and culture.
Main Methods:
- Analysis of 40 reference fungal strains and 120 clinical samples.
- Comparison of EUROArray results with microscopy and/or culture.
- Assessment of analytical sensitivity, specificity, and clinical performance metrics.
Main Results:
- The assay demonstrated 100% analytical sensitivity and 97.1% analytical specificity for fungal identification.
- Clinical samples showed 78.6% sensitivity and 91.7% specificity, detecting cases missed by culture.
- Positive predictive value was 75.9% and negative predictive value was 92.8%.
Conclusions:
- The EUROArray platform provides rapid, broad-spectrum fungal detection, complementing conventional diagnostics.
- The assay identifies non-viable fungi and low fungal loads, improving diagnostic yield.
- Future enhancements could expand the identification panel and optimize sample handling for greater clinical utility.
