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Complement activation by clumping factor and protein A from Staphylococcus aureus strain E 2371.

F Espersen

    Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology
    |April 1, 1985
    PubMed
    Summary
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    Staphylococcus aureus surface proteins, clumping factor and protein A, activate the human complement system. Clumping factor is a more potent activator than protein A, and both activate the alternative complement pathway.

    Area of Science:

    • Immunology
    • Microbiology
    • Biochemistry

    Background:

    • Staphylococcus aureus is a significant human pathogen.
    • Surface proteins of S. aureus can interact with host immune components.
    • Complement activation is a key part of the innate immune response.

    Purpose of the Study:

    • To investigate the complement-activating potential of purified S. aureus surface proteins.
    • To compare the relative potency of different S. aureus surface proteins in complement activation.
    • To determine which complement pathway(s) are activated by these proteins.

    Main Methods:

    • Purification of surface components from Staphylococcus aureus strain E 2371.
    • In vitro testing of complement activation in normal human serum.

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  • Analysis of complement activation using serum chelated with ethylene-glycerol-tetra-acetic acid and magnesium chloride.

    • Main Results:

    • Purified staphylococcal clumping factor and protein A activated complement in normal human serum.
    • Fibronectin-binding protein did not show complement-activating activity.
    • Clumping factor demonstrated greater potency than protein A in inducing complement consumption at identical molar concentrations.
    • Both clumping factor and protein A activated the alternative complement pathway.

    Conclusions:

    • Staphylococcal clumping factor and protein A are potent activators of the human complement system.
    • These proteins primarily activate the alternative complement pathway.
    • Understanding these interactions is crucial for developing strategies against S. aureus infections.