Related Experiment Video
Updated: May 20, 2025

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
FungID: Innovative Fungi Identification Method with Chromogenic Profiling of Colony Color Patterns
John Pouris1, Konstantinos Konstantinidis2, Ioanna Pyrri3
1Laboratory of Molecular Microbiology and Immunology, Department of Biomedical Sciences, University of West Attica, 12243 Athens, Greece.
Abstract:
Fungi play crucial roles in many ecosystems; however, traditional identification methods are often time- and labor-intensive. In this study, we introduce FungID, a pilot and novel deep learning algorithm, alongside its user-friendly software implementation, developed by analyzing various fungal species for identification based on chromogenic profiling of colony color patterns via a Convolutional Neural Network. Training and testing FungID upon a set of 269 images showed remarkable performance in terms of model robustness and classification efficacy. These findings demonstrate that FungID offers a potential method for rapid and reliable identification of fungal species through chromogenic profiling, providing additional tools to conventional techniques being employed in the fields of health, microbiology, biotechnology, and more. Our research underscores the promising role of deep learning algorithms in enhancing the understanding of the taxonomy and ecological functions of fungi that can be grown in pure cultures, while also emphasizing the importance of carefully assessing the scope and limitations of these methods.
Related Concept Videos
Karyotyping
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

