A novel glass chip based lateral flow immunoassay of albumin

Lateral flow immunoassays typically rely on optical tests conducted on paper strips. However, the 3D matrix of paper embedded with optical nanoparticles often limits detection sensitivity and the ability of detection instruments to capture signals. This study introduces a novel approach using a glass chip-based lateral flow immunoassay, with albumin as a typical biomarker for detection, enabling the presence of the signal on a flat surface. Compared with traditional paper-based immunoassay, glass-based lateral flow immunoassay has achieved a uniform distribution pattern for albumin detection, lowered the limit of detection from 100 ng/mL to 1 ng/mL, and reduced detection time through an improved liquid mobility system. The glass-based method also shortens the detection time by 28.5% to 147.8 s compared to the traditional method. This research presents a new methodology for lateral flow immunoassays that can be applied to a wide range of biomarkers, with potential benefits for various medical and environmental applications.