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FGFR3 gene mutations screening in non-muscle invasive bladder cancer (NMIBC) in the Tunisian population

  • 0Urology Department, Charles Nicolle Hospital, Faculty of Medicine, Tunis El Manar University, Tunis, 1006, Tunisia.
Molecular Biology Reports +

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March 26, 2025

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March 26, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Fibroblast Growth Factor Receptor (FGFR3) gene mutations are common in non-muscle invasive bladder tumors (NMIBC). These FGFR3 mutations appear to be a favorable prognostic indicator for NMIBC patients in Tunisia.

Area Of Science

  • Oncology
  • Genetics
  • Urology

Background

  • Fibroblast Growth Factor Receptor (FGFR3) gene mutations are implicated in non-muscle invasive bladder tumors (NMIBC).
  • The prognostic significance of FGFR3 mutations in the Tunisian population for NMIBC is not well-established.
  • This study investigates the impact of FGFR3 mutations on NMIBC recurrence, progression, and survival in Tunisia.

Purpose Of The Study

  • To determine the prognostic value of FGFR3 gene mutations in non-muscle invasive bladder tumors (NMIBC).
  • To assess the association between FGFR3 mutations and clinical outcomes including recurrence, progression, and survival.
  • To analyze FGFR3 mutation frequency and types in a Tunisian NMIBC cohort.

Main Methods

  • Retrospective analysis of 42 NMIBC patient samples.
  • DNA extraction from formalin-fixed paraffin-embedded tissues.
  • Polymerase Chain Reaction (PCR) and Sanger sequencing for FGFR3 hot-spot mutations (exons 7 and 15).
  • Kaplan-Meier analysis for overall survival (OS) and disease-specific survival (DSS).

Main Results

  • FGFR3 mutations were detected in 68% of NMIBC tumors, with 6 novel variants identified.
  • No statistically significant correlation was found between FGFR3 mutations and decreased OS or DSS.
  • Tumor characteristics like solid appearance, calcifications, and stage were associated with FGFR3 mutation presence.

Conclusions

  • FGFR3 mutations are prevalent in the studied NMIBC cohort.
  • FGFR3 mutations may represent a favorable prognostic factor for NMIBC in the Tunisian population.
  • Further research is warranted to confirm the favorable prognostic role of FGFR3 mutations in NMIBC.

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