Mannanase from Lactic Acid Bacteria Pediococcus acidilactici: Production Optimization, Purification, and Application
Jingjun Shi1, Xi Chen1, Ruoxi Yang1
1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, Heilongjiang, People's Republic of China.
Applied Biochemistry and Biotechnology
|March 26, 2025
Summary
Pediococcus acidilactici HDM2 produces enhanced mannanase, a safe enzyme for food applications. Optimization increased enzyme yield by 39.3%, showing promise for fruit juice clarification.
Area of Science:
- Microbiology
- Enzymology
- Biotechnology
Background:
- Pediococcus acidilactici is a safe lactic acid bacteria species, recognized as a valuable source of microbial mannanase.
- Mannanase enzymes are crucial for various industrial applications, including food processing.
Purpose of the Study:
- To isolate and identify a novel Pediococcus acidilactici strain (HDM2) for mannanase production.
- To optimize mannanase production using response surface methodology (RSM).
- To evaluate the efficacy of the purified mannanase in fruit juice clarification.
Main Methods:
- Strain identification through morphological, physiological, biochemical, and molecular (16S rDNA, rpoA) analyses.
- Two-step RSM to determine optimal conditions (glucose concentration, initial pH) for mannanase production.
- Enzyme purification and characterization (molecular weight determination).
- Application in fruit juice clarification and comparison with commercial mannanase.
Main Results:
- Pediococcus acidilactici HDM2 was successfully isolated and identified.
- Optimized conditions (17.5 g/L glucose, pH 4.6) yielded a 39.3% increase in mannanase activity (97.9 U/mL).
- Enlarged clearance zone (2.7 to 4.5 cm) confirmed enhanced enzyme production.
- Purified mannanase (30 KDa) showed superior clarifying efficiency in six of eight fruit juices compared to commercial enzymes.
Conclusions:
- RSM effectively optimized mannanase production in Pediococcus acidilactici HDM2.
- The enhanced mannanase demonstrates significant potential for industrial applications, particularly in food-level processes like fruit juice clarification.
- P. acidilactici HDM2 represents a promising source of safe and efficient mannanase for biotechnological use.
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