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Related Experiment Video

Updated: May 20, 2025

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
08:25

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

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Assessing the exercise-related kinetics of circulating cell-free DNA, circulating tumour DNA, DNase I activity and

Elmo W I Neuberger1, Alexandra Brahmer1, Tobias Ehlert1

  • 1Department of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University Mainz, Mainz, Germany.

Experimental Physiology
|March 26, 2025
PubMed
Summary
This summary is machine-generated.

Acute exercise increases cell-free DNA (cfDNA) and neutrophil cytokines in cancer patients and controls. This study explored cfDNA, ctDNA, and cytokine kinetics following exercise in cancer patients versus healthy individuals.

Keywords:
cancercirculating cell‐free DNAcirculating tumour DNAcytokineexerciseneutrophilsolid tumour

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Area of Science:

  • Oncology
  • Exercise Physiology
  • Biomarker Research

Background:

  • Circulating cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), and inflammatory cytokines are crucial in oncology.
  • The impact of acute exercise on cfDNA levels in cancer patients remains unexplored.

Purpose of the Study:

  • To investigate the kinetics of cfDNA, ctDNA, and cytokines after an incremental exercise test in cancer patients compared to healthy controls.
  • To assess the feasibility and safety of cardiopulmonary exercise testing in cancer patients for biomarker analysis.

Main Methods:

  • A pilot study involving 12 cancer patients and 6 healthy controls performing a cardiopulmonary bicycle test.
  • Blood samples collected pre-exercise, immediately post-exercise, and 90 minutes post-exercise.
  • Analysis of cfDNA, ctDNA (KRAS mutations), DNase I activity, and various cytokine levels.

Main Results:

  • cfDNA levels increased post-exercise and returned to baseline within 90 minutes in all participants.
  • cfDNA concentrations and DNase I activity correlated in controls but not in cancer patients.
  • Exercise triggered significant increases in neutrophil-associated markers (MPO, calprotectin, NGAL).
  • ctDNA levels decreased post-exercise in the single patient where it was detected.

Conclusions:

  • Cardiopulmonary exercise testing is safe and feasible in cancer patients.
  • Acute exercise appears to trigger the release of cfDNA and neutrophil-derived cytokines in cancer patients.
  • These findings suggest potential exercise-induced biomarker dynamics in cancer.