Bright fluorescent biocompatible Magnozyme nanoclusters for brain-cellin-vivo live imaging

Area of Science:

• Materials Science
• Nanotechnology
• Biomedical Engineering

Background:

• Developing novel nanomaterials for biological imaging is crucial.
• Existing probes often face limitations in stability, toxicity, or spectral range.

Purpose of the Study:

• To synthesize and characterize multifluorescent, water-dispersible magnesium nanoclusters (Magnozyme).
• To evaluate Magnozyme's potential as a cellular imaging probe in vitro and in vivo.

Main Methods:

• Simple and economical synthesis of 4 nm magnesium nanoclusters.
• Characterization of optical properties (emission/excitation wavelengths, quantum yield) and stability (pH, ionic strength).
• Assessment of cell viability (U-87 MG cell lines) and in vivo toxicity in mice.

Main Results:

• Magnozyme exhibited strong blue, green, and red fluorescence with high photostability and broad pH/ionic strength tolerance.
• Demonstrated 95% cell viability and significant cytoplasmic accumulation in brain cells.
• Confocal Z-stack imaging confirmed cellular depth penetration.
• In vivo studies showed no toxicity and excellent staining ability.

Conclusions:

• Magnozyme is a stable, non-toxic, multifluorescent nanomaterial suitable for biological imaging.
• Its properties support its use as a versatile probe for cellular imaging and labeling in vitro and in vivo.