Metabolic modelling of anaerobic amino acid uptake and storage by fermentative polyphosphate accumulating organisms

Existing enhanced biological phosphorus removal (EBPR) models do not fully describe the metabolism of fermentative polyphosphate accumulating organisms (fPAOs), particularly under mixed substrate conditions representative of fermentation-enhanced EBPR (F-EBPR) processes. This study presents a steady-state metabolic model integrating anaerobic amino acid (AA) fermentation and storage processes in fPAOs. The model identifies key metabolic interactions underlying fPAO metabolism, prioritising substrate accumulation over fermentation. This results in significant changes to ATP and reduction-oxidation (redox) flows as compared to when relying on previous AA fermentation models typically used to describe fPAO metabolism, with medium-chain-length (MCL) polyhydroxyalkanoate (PHA) formation and polyphosphate (polyP) consumption acting as important electron and energy management mechanisms, respectively. Succinate, rather than volatile fatty acids (VFAs), was identified as the more likely synergetic substrate between fermentative and conventional PAOs (cPAOs) under these conditions. Moreover, conditions favourable of VFA efflux by fPAOs may also favour a shift away from a polyP accumulation to a fermentation dominant metabolism. Further work is required to verify the role of MCL-PHA fractions, alongside the contribution of free intracellular AA accumulation as compared to polymers such as cyanophycin or polyglutamate on fPAO metabolism. This metabolic model provides a framework for better understanding the role of fPAOs and their interactions with cPAOs within EBPR processes, informing future modelling and optimisation of F-EBPR systems.