Influence of the ion-exchange functional groups on protein anion-exchange chromatography

Several anion-exchange resins with different ion-exchange functional groups were prepared and evaluated with a protein mixture commercially available for evaluating anion-exchange resin (Myoglobin, Conalbumin, Ovalbumin, Soybean trypsin inhibitor). These functional groups include the commonly used diethyl amino group, two diamines, and the dibutyl amino group. The elution behaviors of the diamines are almost identical to that of the diethyl amino group, while dibutyl amino group provided significantly different elution behaviors.