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Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
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Spermidine mitigates blue-light-induced corneal injury by alleviating oxidative stress and restoring autophagy

Xiang Li1, Jiayun Ge1, Jiru Zhu1

  • 1Department of Ophthalmology, The First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang Province, China.

Experimental Eye Research
|March 26, 2025
PubMed
Summary
This summary is machine-generated.

Spermidine protects the cornea from blue light damage by enhancing cell repair and antioxidant defenses. Topical spermidine eye drops promote healing and maintain eye health against photodamage.

Keywords:
AutophagyBlue lightCorneal epitheliumMetabolomicsOxidative stressSpermidine

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Blue light (BL) exposure causes corneal photodamage, leading to epithelial injury and impaired wound healing.
  • Metabolomic analysis revealed spermidine downregulation in corneas affected by BL exposure.
  • Autophagy and antioxidant response pathways are implicated in BL-induced corneal damage.

Purpose of the Study:

  • To investigate the protective effects of spermidine against blue light-induced corneal photodamage.
  • To evaluate spermidine's role in cellular processes like proliferation, mitochondrial function, oxidative stress, and autophagy.
  • To assess the therapeutic potential of topical spermidine eye drops in a rabbit model.

Main Methods:

  • Rabbit corneas were exposed to artificial blue light for six weeks.
  • In vitro studies used human corneal epithelial cells exposed to blue light.
  • Metabolomic analysis, cell viability assays, colony formation assays, and assessments of mitochondrial function, oxidative stress, and autophagy were performed.
  • Topical spermidine eye drops were administered to rabbits under blue light exposure.

Main Results:

  • Blue light exposure induced corneal epithelial injury and delayed wound healing, correlating with decreased spermidine levels.
  • Spermidine treatment in vitro reduced cell death, enhanced proliferation and colony formation, and ameliorated mitochondrial dysfunction, oxidative stress, and autophagy impairment.
  • Topical spermidine eye drops reduced corneal epithelial injury in vivo, promoting cell proliferation and wound healing.

Conclusions:

  • Spermidine exhibits significant protective efficacy against blue light-induced corneal photodamage.
  • Spermidine supports corneal epithelial health by enhancing antioxidant capacity, autophagy, and cellular repair mechanisms.
  • Topical spermidine represents a promising therapeutic strategy for managing blue light-related eye conditions.