Butylphthalide identified via Samul-tang-induced transcriptomic signatures improves oocyte quality in aged mice

Area of Science:

• Reproductive biology
• Pharmacology
• Traditional Chinese Medicine

Background:

• Samul-tang (SM) is a traditional herbal prescription used for gynecological conditions, including infertility.
• Previous research indicated SM improves ovarian reserves and oocyte production in aged mice, potentially via Ras signaling.
• The specific bioactive compounds in SM responsible for these effects remain unidentified.

Purpose of the Study:

• To identify the active compounds in Samul-tang (SM) that contribute to its therapeutic effects on age-related decline in ovarian function.
• To investigate the potential of identified compounds in improving ovarian reserve and mature oocyte production.

Main Methods:

• An in vivo mouse model of aging was used to evaluate the effects of SM and its components.
• Combinations of known SM ingredients, including butylphthalide and oleanolic acid, were tested for their impact on oocyte production.
• The efficacy of individual compounds in mimicking SM's effects on ovarian reserve was assessed.

Main Results:

• The combination of butylphthalide and oleanolic acid replicated the positive effects of SM on mature oocyte production in aged mice.
• Butylphthalide was identified as a key active compound responsible for SM's ability to enhance ovarian reserve.
• The study demonstrated a novel strategy for identifying active components in multi-component herbal medicines.

Conclusions:

• Butylphthalide is a significant bioactive compound in Samul-tang (SM) that mitigates age-related decline in ovarian function.
• This finding offers a targeted approach for developing treatments for age-related female infertility.
• The study highlights a new method for dissecting the active components of complex herbal remedies.