Music of Different Tones Maintains Intestinal Immunity by Regulating the Intestinal Barrier and Intestinal Microbiota

Area of Science:

• Microbiology
• Immunology
• Animal Science

Background:

• Music exposure is a known environmental factor influencing animal health.
• The specific impact of musical tones on the intestinal barrier and microbiota remains under investigation.

Purpose of the Study:

• To investigate the effects of different tones of Mozart K.448 on the intestinal barrier and microbiota in Kunming white mice.
• To determine if specific musical tones can modulate intestinal health parameters.

Main Methods:

• 100 Kunming white mice were divided into a control group and three groups exposed to Mozart K.448 in D, A, and G tones.
• Mice received musical stimulation from 1 to 63 days of age.
• Analysis included gene expression of intestinal barrier markers, intestinal permeability assays (DAO, D-lactate), HSP60 levels, immune markers (IgA, IgG), and gut microbiota composition.

Main Results:

• Music exposure significantly increased mRNA expression of intestinal barrier genes (Claudin-1, Claudin-12, ZO-2, Mucin2, ZO-1, Claudin-5), with the D tone showing the highest levels.
• The D tone group exhibited significantly lower intestinal mucosal permeability (DAO, D-lactate) and ileum HSP60 mRNA levels.
• Music stimulation elevated IgA and IgG expression, particularly in the D tone group, and increased the abundance of beneficial bacteria like Lactobacillus and Sporosarcina.

Conclusions:

• Mozart K.448 strengthens intestinal barrier function, reduces permeability, and enhances intestinal immunity in mice.
• Music, especially in the D tone, promotes the colonization of beneficial gut microbiota.
• Musical tones represent a significant factor in modulating intestinal health and immunity.