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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Neurogenesis and Regeneration of Nervous Tissue01:15

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In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
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Related Experiment Video

Updated: May 20, 2025

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

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Notch Is Required for Neural Progenitor Proliferation During Embryonic Eye Regrowth.

Dylan J Guerin1, Belen Gutierrez1, Baoyi Zhang1

  • 1School of Life Sciences, University of Nevada, Las Vegas, NV 89154, USA.

International Journal of Molecular Sciences
|March 27, 2025
PubMed
Summary
This summary is machine-generated.

The Notch signaling pathway is essential for embryonic eye regrowth in Xenopus laevis, regulating retinal progenitor cell proliferation. Inhibition of Notch1 blocks regrowth, while its activation rescues defects caused by V-ATPase inhibition.

Keywords:
NotchRPCsV-ATPaseXenopuseyeneural stem cellsregenerationregrowthretina

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Area of Science:

  • Developmental Biology
  • Regenerative Medicine
  • Cell Signaling

Background:

  • Tissue regeneration is controlled by signaling pathways.
  • The Notch signaling pathway is crucial for development and regeneration.
  • The role of Notch in Xenopus laevis embryonic eye regrowth is not well understood.

Purpose of the Study:

  • To investigate the role of Notch signaling in Xenopus laevis embryonic eye regrowth.
  • To determine if Notch1 regulates retinal progenitor cell proliferation and differentiation during eye regeneration.

Main Methods:

  • Chemical and molecular inhibition of Notch1.
  • Temporal inhibition studies.
  • Overexpression of Notch intracellular domain (NICD).
  • Loss-of-function studies for Notch1 and V-ATPase.

Main Results:

  • Notch1 is required for Xenopus laevis embryonic eye regrowth.
  • Notch1 inhibition reduces retinal progenitor cell proliferation but not differentiation.
  • Notch1 function is critical during the initial phase of regrowth.
  • Notch1 loss-of-function mimics V-ATPase inhibition effects on cell proliferation.
  • NICD overexpression rescues eye regrowth defects caused by V-ATPase inhibition.

Conclusions:

  • The Notch signaling pathway plays a vital role in embryonic eye regeneration in Xenopus laevis.
  • Notch1 signaling is essential for inducing retinal progenitor cell proliferation post-injury.
  • Notch1 and V-ATPase pathways interact to regulate cell proliferation during eye regrowth.