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Deciphering of SOX9 Functions in Pancreatic Cancer Cells.

Kirill Kashkin1, Liya Kondratyeva1, Eugene Kopantzev1

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia.

International Journal of Molecular Sciences
|March 27, 2025
PubMed
Summary

SOX9 knockdown in pancreatic cancer cells altered gene expression, impacting cell signaling, differentiation, and cell cycle processes. This reveals SOX9

Keywords:
SOX9gene signatureknockdownpancreatic cancerpathway analysissiRNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • SOX9 is a master transcription regulator crucial for development and differentiation.
  • SOX9 overexpression is linked to pancreatic cancer progression and serves as a prognostic marker.

Purpose of the Study:

  • To investigate the functional role of SOX9 in pancreatic cancer.
  • To analyze the global gene expression changes following SOX9 knockdown.

Main Methods:

  • SOX9 knockdown was performed in pancreatic cancer cell lines (COLO357, PANC-1) using RNA interference (siRNA).
  • Full-transcriptome analysis (RNA sequencing) was conducted on siRNA-transfected cells.
  • Pathway enrichment analysis was used to identify altered molecular pathways.

Main Results:

  • SOX9 knockdown activated pathways in cellular signaling, differentiation, transcription, and methylation.
  • Genes involved in the cell cycle and apoptosis were suppressed post-SOX9 knockdown.
  • Expression of transcription factors, EMT markers, oncogenes, tumor suppressors, and drug resistance genes were altered.

Conclusions:

  • SOX9 plays a significant role in regulating key cellular processes in pancreatic cancer.
  • Understanding SOX9's impact on gene expression provides insights into pancreatic cancer biology and potential therapeutic targets.
  • Further research with diverse models is necessary to fully elucidate SOX9's prognostic significance.