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Related Experiment Video

Updated: May 20, 2025

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Genomic Prediction for Germplasm Improvement Through Inter-Heterotic-Group Line Crossing in Maize.

Dehe Cheng¹, Jinlong Li¹, Shuwei Guo¹

¹State Key Laboratory of Maize Bio-Breeding, National Maize Improvement Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.

International Journal of Molecular Sciences

|March 27, 2025

Summary

This study shows inter-heterotic-group crossing effectively creates new maize heterotic groups for germplasm improvement. Genomic selection accurately predicts performance in these new populations, aiding breeding efficiency.

Keywords:

genomic selection germplasm improvement inter-heterotic-group maize

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Last Updated: May 20, 2025

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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Published on: January 3, 2014

Area of Science:

Plant breeding and genetics
Genomics and bioinformatics
Agricultural science

Background:

Maize (Zea mays L.) germplasm improvement relies heavily on intra-heterotic-group crossing.
Inter-heterotic-group crossing offers potential for novel germplasm but lacks systematic application.
Developing new heterotic groups is crucial for sustained genetic gains in maize.

Purpose of the Study:

To systematically develop and evaluate a connected segregating population through inter-heterotic-group line crossing (CSPIC).
To assess the accuracy of genomic selection (GS) for predicting traits within and across populations in CSPIC.
To explore the feasibility of creating new heterotic groups via inter-heterotic-group crossing for maize breeding.

Main Methods:

Developed a CSPIC using five inbred lines from four heterotic groups, creating 5 subpopulations with 535 doubled haploid (DH) lines and 1568 hybrids.
Evaluated phenotypic traits including plant height, ear height, days to anthesis, and days to silking.
Assessed genomic selection prediction accuracy within DH and hybrid populations, and across different cross-population scenarios.

Main Results:

Significant genetic variation was observed, with superior phenotypes in several DH populations.
10.8% of hybrids in POP5/C229 exceeded the high-yielding check hybrid ND678.
Genomic selection achieved average hybrid yield prediction accuracies of 0.41 within populations and demonstrated strong cross-population prediction, correlated with shared polymorphisms.

Conclusions:

Inter-heterotic-group crossing is a feasible strategy for creating novel maize heterotic groups and improving germplasm.
Genomic selection is effective for predicting performance in new populations derived from inter-heterotic-group crosses.
The study provides a systematic approach and valuable insights for utilizing inter-heterotic-group crossing in maize breeding programs.