Formulation and In Vitro Characterization of Granulocyte-Colony-Stimulating-Factor-Loaded Liposomes: Future...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Reproductive Medicine

Background:

• Drug delivery systems improve therapeutic efficacy and reduce side effects.
• Liposomes are biocompatible, biodegradable carriers with low toxicity.
• Granulocyte colony-stimulating factor (G-CSF) shows promise in treating infertility and endometritis by enhancing endometrial receptivity.

Purpose of the Study:

• To prepare and characterize G-CSF-loaded liposomes for enhanced drug delivery.
• To evaluate the physical stability and cytocompatibility of the G-CSF liposomal formulation.

Main Methods:

• Liposomes were prepared using passive loading via thin-film hydration.
• Particle size, PDI, and zeta potential were measured using dynamic and electrophoretic light scattering.
• Encapsulation efficiency was quantified using HPLC, and morphology was assessed via cryogenic transmission electron microscopy.
• Cytotoxicity was evaluated on human dermal fibroblasts using an MTT assay.

Main Results:

• G-CSF-loaded liposomes exhibited an average particle size of 161.9 nm, PDI of 0.261, and zeta potential of +2.09 mV.
• High physical stability was observed during long-term storage.
• Encapsulation efficiency reached 52.37% with no significant cytotoxicity detected.
• A dose-dependent effect on fibroblast viability was noted.

Conclusions:

• Successful preparation of stable and non-cytotoxic G-CSF-loaded liposomes was confirmed.
• These liposomes demonstrate potential for improved G-CSF delivery in biomedical applications.
• Further in vivo studies are necessary to ascertain the safety and efficacy of these formulations.