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Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Spermidine as a Potential Protective Agents Against Poly(I:C)-Induced Immune Response, Oxidative Stress, Apoptosis,

Yujun Tang1, Hao Li1, Yutian Zeng1

  • 1College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

International Journal of Molecular Sciences
|March 27, 2025
PubMed
Summary
This summary is machine-generated.

Spermidine (SPD) protects male yak Leydig cells from dsRNA virus damage, reducing inflammation, oxidative stress, and apoptosis. This enhances testosterone production, offering a potential treatment for viral-induced infertility in yaks.

Keywords:
Leydig cellimmune responseoxidative stressspermidineyak

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Area of Science:

  • Veterinary Immunology
  • Reproductive Biology
  • Molecular Toxicology

Background:

  • Viral infections in male yaks cause infertility via Leydig cell apoptosis, immune activation, and oxidative stress.
  • Reduced testosterone production is a key consequence of these viral infections.

Purpose of the Study:

  • To investigate the protective effects of spermidine (SPD) against dsRNA virus-induced damage in yak Leydig cells (LCs).
  • To elucidate the molecular mechanisms underlying SPD's therapeutic potential in restoring male yak fertility.

Main Methods:

  • Yak testicular LCs were treated with Poly(I:C) to model viral infection.
  • Immune response, apoptosis, oxidative stress, and testosterone levels were assessed in LCs with or without SPD.
  • Transcriptomic sequencing was employed to analyze molecular changes.

Main Results:

  • Poly(I:C) induced LC dysfunction, decreasing testosterone and increasing immune response, oxidative stress, and apoptosis.
  • SPD treatment significantly alleviated immune response and oxidative stress.
  • SPD inhibited apoptosis and restored testosterone production in LCs.

Conclusions:

  • SPD effectively mitigates dsRNA virus-induced immune response, oxidative stress, and apoptosis in yak LCs.
  • SPD demonstrates potential for restoring testosterone production and enhancing male yak fertility post-viral infection.
  • Transcriptomic analysis revealed SPD's role in regulating key genes involved in inflammation and apoptosis.