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Detection of Histone Modifications in Plant Leaves
Published on: September 23, 2011
DNA Demethylase ROS1 Interferes with DNA Methylation and Activates Stress Response Genes in Plants Infected with Beet
Taicheng Jin1, Yushuo Li1, Xu Sun1
1The School of Life Sciences, Jilin Normal University, Siping 136000, China.
Abstract:
DNA methylation is one mechanism of epigenetic regulation in plants. Small interfering RNAs (siRNAs) targeted endogenous genes and caused the promoters to be hypermethylated, namely RNA-directed DNA methylation (RdDM). Repressor of silencing 1 (ROS1) is an active DNA demethylase involved in the regulation of DNA methylation. This study indicates that ROS1-mediated DNA demethylation plays important roles in regulating the expression of these stress response genes and in response to biotic stresses. Further experiments confirmed that the expression level of the ROS1 gene was significantly upregulated in A. thaliana plants infected with beet severe curly top virus (BSCTV). Moreover, the DNA sequencing results demonstrated that ROS1 interferes with DNA methylation of repeat regions in the promoters of ACD6, GSTF14, and ACO3 in A. thaliana plants infected with BSCTV. These findings reveal the epigenetic mechanisms by which ROS1 regulates the expression of the stress response genes, thereby improving the adaptability of plants to biotic stresses.
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