Related Experiment Video

Updated: May 20, 2025

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

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NCKAP1 Inhibits the Progression of Renal Carcinoma via Modulating Immune Responses and the PI3K/AKT/mTOR Signaling

Xin Zhang1, Jianqing Ye2, Lixiang Sun1

  • 1Central Laboratory, Zhongshan Hospital (Xiamen), Fudan University, Xiamen 361015, China.

International Journal of Molecular Sciences
|March 27, 2025
PubMed

Insights

Nck-associated protein 1 (NCKAP1) is crucial in kidney cancer. Lower NCKAP1 levels correlate with poor prognosis and impact immune cell infiltration, suggesting it as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Nck-associated protein 1 (NCKAP1) is vital for cellular functions and cancer progression.
  • Its role in renal cancer development and immune regulation remains unclear.

Purpose of the Study:

  • To investigate NCKAP1's function in renal cancer progression and immune modulation.
  • To explore NCKAP1 as a potential biomarker and therapeutic target in kidney cancer.

Main Methods:

  • Pancancer analysis of NCKAP1 expression and variations using UALCAN and cBioPortal.
  • Assessment of immune cell infiltration via ssGSEA and single-cell RNA sequencing.
  • RNA sequencing of NCKAP1-overexpressing cells and pathway analysis (PI3K/AKT/mTOR).

Main Results:

  • NCKAP1 expression varies across cancers; reduced levels in renal cancer correlate with poorer patient outcomes.
  • NCKAP1 expression patterns identified in distinct renal cancer cell lineages.
  • Significant correlation found between NCKAP1 and immune cell infiltration in the kidney tumor microenvironment.
  • NCKAP1 suppresses renal cancer cell growth and tumor development via the PI3K/AKT/mTOR pathway.

Conclusions:

  • NCKAP1 plays a significant role in renal cancer progression and immune response.
  • NCKAP1 acts as a tumor suppressor in kidney cancer.
  • NCKAP1 is a promising predictive marker and therapeutic target for renal cancer.
Keywords:
NCKAP1immunotherapypancancerrenal cancer

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