Related Experiment Video
Updated: May 20, 2025

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
Published on: July 16, 2017
Impacts of Land Use on Soil Nitrogen-Cycling Microbial Communities: Insights from Community Structure, Functional
1Heilongjiang Province Key Laboratory of Cold Region Wetland Ecology and Environment Research, Harbin University, Harbin 150086, China.
Abstract:
This study investigates the effects of different land-use types (forest, arable land, and wetland) on key soil properties, microbial communities, and nitrogen cycling in the Lesser Khingan Mountains. The results revealed that forest (FL) and wetland (WL) soils had significantly higher soil organic matter (SOM) content compared with arable land (AL), with total phosphorus (TP) being highest in FL and available nitrogen (AN) significantly higher in WL. In terms of enzyme activity, AL and WL showed reduced activities of ammonia monooxygenase (AMO), β-D-glucosidase (β-G), and β-cellobiosidase (CBH), while exhibiting increased N-acetyl-β-D-glucosaminidase (NAG) activity, highlighting the impact of land use on nitrogen dynamics. WL also exhibited significantly higher microbial diversity and evenness compared with FL and AL. The dominant bacterial phyla included Actinobacteriota, Proteobacteria, and Acidobacteriota, with Acidobacteriota being most abundant in FL and Proteobacteria most abundant in WL. Network analysis showed that AL had the most complex and connected microbial network, while FL and WL had simpler but more stable networks, suggesting the influence of land use on microbial community interactions. Regarding nitrogen cycling genes, AOA-amoA was most abundant in AL, while AOB-amoA was significantly enriched in FL, reflecting the influence of land use on ammonia oxidation. These findings highlight how land-use types significantly affect soil properties, microbial community structures, and nitrogen cycling, offering valuable insights for sustainable land management.
More Related Videos
10:31Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
10:16Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
Published on: January 16, 2014
Related Concept Videos
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The Nitrogen Cycle
The Roles of Bacteria and Fungi in Plant Nutrition
The Soil Ecosystem
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...