La Jolla Virus: The Pathology and Transmission in Its Host Drosophila suzukii

Drosophila suzukii, commonly known as spotted-wing drosophila, has emerged as a highly destructive pest in global fruit and wine production. The effectiveness of chemical control is significantly compromised by rapid resistance development and a limited range of insecticide options. Biological control presents a promising sustainable alternative. Our previous work suggested the La Jolla Virus (LJV) as a suitable candidate for the development of an insect virus-based control option. Here, we characterized the natural transmission and pathology of the virus. We tested various modes of horizontal transmission, including airborne, venereal and oral, and fecal routes. To understand LJV pathology in infected flies, we studied feeding behavior and demonstrated changes in food absorption compared to non-infected flies. We also investigated the impact on fecundity and egg-to-adult success rate. Altogether, these results collectively improve our understanding of LJV transmission in natural populations and the implication of infected flies in food ingestion and overall fitness.