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Related Experiment Video

Updated: May 20, 2025

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
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Preclinical Testing of 3D Printed, Cell Loaded Hydrogel Based Corneal Substitutes on Rabbit Model.

Deniz Basoz1,2, Aslihan Akalinli1,3, Senem Buyuksungur4

  • 1Acibadem Mehmet Ali Aydinlar University (ACU), Biomaterials R&D Center, Istanbul, 34752, Türkiye.

Macromolecular Bioscience
|March 27, 2025
PubMed
Summary
This summary is machine-generated.

3D printed methacrylated gelatin (GelMA) implants show promise for corneal stroma repair. These biomaterial implants degraded over 90 days in rabbits, promoting healthy tissue regeneration and reducing immune response.

Keywords:
3D printingbiomaterialscorneal stromahydrogelstissue engineering

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Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Tissue Engineering

Background:

  • Corneal stroma injuries cause vision loss, with donor tissue transplantation being the current standard treatment.
  • Limitations of corneal allografts include donor tissue scarcity, disease transmission risks, and immune rejection.
  • Biomaterial implants offer a potential alternative to donor tissues for corneal repair.

Purpose of the Study:

  • To develop and evaluate 3D printed methacrylated gelatin (GelMA) implants as substitutes for corneal stroma.
  • To assess the physical properties and transparency of GelMA implants.
  • To investigate the in vivo performance and biocompatibility of cell-loaded GelMA implants in a rabbit model.

Main Methods:

  • Methacrylated gelatin (GelMA) implants were fabricated using 3D printing.
  • In vitro characterization included printability, water content, mechanical strength, and light transmission.
  • Cell-loaded GelMA implants were implanted in New Zealand white rabbits for 90 days to evaluate in vivo performance.

Main Results:

  • GelMA implants exhibited suitable printability, mechanical strength, and retained 60%-80% light transmission.
  • In vivo, cell-loaded implants showed significant degradation over 90 days.
  • Implantation sites demonstrated successful tissue reorganization, reduced immune response, and restoration of healthy corneal architecture.

Conclusions:

  • 3D printed GelMA implants are a promising biomaterial for corneal stroma regeneration.
  • These implants facilitate tissue remodeling and exhibit good biocompatibility.
  • The developed GelMA implants show potential for clinical application in treating corneal injuries.