Related Experiment Video

Updated: May 20, 2025

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

7.2K

KLF1 Promotes Cardiomyocyte Proliferation and Heart Regeneration Through Regulation of Wnt/β-Catenin Signaling

Yanglin Hao1, Xi Zhang1, Shuan Ran1

  • 1Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 27, 2025
PubMed

Insights

Krüppel-like factor 1 (KLF1) promotes heart regeneration by increasing cardiomyocyte proliferation. Targeting KLF1 offers a new therapeutic strategy for treating heart failure and improving cardiac repair.

Area of Science:

  • Cardiovascular Biology
  • Regenerative Medicine
  • Molecular Cardiology

Background:

  • Heart failure is a major global health concern requiring novel therapeutic strategies.
  • Myocardial regenerative capacity significantly declines postnatally.
  • Krüppel-like factor 1 (KLF1) expression decreases with age, coinciding with reduced heart repair ability.

Purpose of the Study:

  • To investigate the role of Krüppel-like factor 1 (KLF1) in cardiomyocyte proliferation and heart regeneration.
  • To explore KLF1's regulatory mechanisms, including its interaction with the Wnt/β-catenin signaling pathway.
  • To assess the therapeutic potential of targeting KLF1 for heart failure treatment.

Main Methods:

  • In vivo and in vitro studies using neonatal and adult mice.
  • Overexpression and knockout models of KLF1.
  • RNA sequencing (RNA-seq) and ATAC sequencing (ATAC-seq) analyses.
  • Assessment of cardiomyocyte proliferation, myocardial repair, and signaling pathways.

Main Results:

  • KLF1 overexpression enhanced cardiomyocyte proliferation and promoted myocardial repair after infarction in mice.
  • KLF1 knockout diminished these regenerative effects.
  • KLF1's pro-proliferative action is linked to the Wnt/β-catenin pathway, mitochondrial function, and fatty acid metabolism.

Conclusions:

  • KLF1 plays a critical role in promoting cardiomyocyte proliferation and cardiac regeneration.
  • KLF1 represents a promising therapeutic target for heart failure.
  • Understanding KLF1's molecular mechanisms provides new insights into cardiac repair strategies.
Keywords:
KLF1Wnt/β‐catenin signaling pathwaycardiomyocyte proliferation and heart regenerationheart failure

More Related Videos

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells05:38

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Published on: February 12, 2021

4.0K
Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes08:42

Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes

Published on: August 28, 2016

26.5K

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.6K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.2K
Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.2K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.0K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.2K