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Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
Published on: January 30, 2014
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Microglial CARD19 ameliorates post-stroke neuroinflammation by stabilizing mitochondrial cristae.
Yujie Hu1,2, Liwen Zhu1,3, Chao Zhou1,2
1Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu Province, China.
Neural Regeneration Research
|March 27, 2025
Summary
Caspase activation and recruitment domain 19 (CARD19) in microglia protects against neuroinflammation after ischemic stroke. Targeting CARD19 may preserve mitochondria and reduce stroke damage.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Microglia are key immune cells in the brain, activated after ischemic stroke.
- Mitochondrial dysfunction worsens neuroinflammation mediated by microglia post-stroke.
- Caspase activation and recruitment domain 19 (CARD19) is implicated in innate and inflammatory responses.
Purpose of the Study:
- To investigate the role of CARD19 in microglial biology and its impact on ischemic stroke.
- To determine CARD19 expression levels in microglia following ischemic stroke.
Main Methods:
- Analysis of spatial transcriptomic sequencing data from ischemic brain tissue.
- In vitro studies using microglial activation models.
- Conditional knockdown of Card19 in a mouse model of ischemic stroke.
- Mitochondrial ultrastructure and function analysis.
Main Results:
- CARD19 expression was significantly elevated in microglia within the ischemic penumbra.
- Microglial CARD19 deficiency exacerbated neuroinflammation and worsened neurological outcomes in mice.
- CARD19 localizes to mitochondria, promoting intermembrane bridge assembly.
- CARD19 deficiency led to mitochondrial cristae damage and an exaggerated inflammatory response.
Conclusions:
- CARD19 plays a protective role in microglia following ischemic stroke.
- CARD19 is crucial for maintaining mitochondrial integrity and function in microglia.
- Targeting CARD19 may represent a novel therapeutic strategy to reduce neuroinflammation and ischemic damage.
