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Updated: Apr 28, 2026

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Published on: March 9, 2014
Preparation of Chemocompetent Agrobacterium tumefaciens Cells
Samuel Parra1, Claudia Stange Klein2
1Centro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Abstract:
Agrobacterium tumefaciens, a soilborne bacterium known for its natural ability to transfer genetic material into plant cells, revolutionized molecular biology and plant genetic engineering. To harness this transformative potential, researchers require competent Agrobacterium cells capable of efficiently delivering desired genetic constructs into plant hosts. Here, we describe a comprehensive and inexpensive method for obtaining Agrobacterium chemocompetent cells, focusing on key aspects such as cell preparation, transformation efficiency, and optimization.

