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Related Experiment Video

Updated: Jul 11, 2026

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Published on: June 9, 2016

Protocol for biomimetic tumoroid models by plastic compression using centrifugation.

Sam Devereaux¹, Ashley Lam¹, Anuja Upadhyay¹

¹UCL Centre for 3D Models of Health and Disease, UCL Division of Surgery and Interventional Science, Faculty of Medical Sciences, Charles Bell House, University College London, 43-45 Foley Street, London W1W 7TY, UK.

|March 27, 2025

Summary

We developed a new protocol using plastic compression and centrifugation to create biomimetic tumoroid models. This method enhances collagen density and mechanical properties for better tumor microenvironment research.

Keywords:

Cancer Cell-based Assays Tissue Engineering

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Last Updated: Jul 11, 2026

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

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Published on: June 9, 2016

A Melanoma Patient-Derived Xenograft Model

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Published on: May 20, 2019

Tumor Spheroid Fabrication and Encapsulation in Polyethylene Glycol Hydrogels for Studying Spheroid-Matrix Interactions

Tumor Spheroid Fabrication and Encapsulation in Polyethylene Glycol Hydrogels for Studying Spheroid-Matrix Interactions

Published on: September 22, 2023

Area of Science:

Biomedical Engineering
Cancer Research
Biomaterials Science

Background:

Tumor microenvironment research requires advanced models.
Current tumoroid models lack mechanical and structural complexity.
Biomimetic engineering offers a path to more accurate models.

Purpose of the Study:

To present a novel protocol for engineering biomimetic tumoroid models.
To utilize plastic compression via centrifugation for model fabrication.
To enhance the mechanical properties and collagen density of tumoroid models.

Main Methods:

Protocol for generating multi-compartment tumor-stroma models.
Mixing cells into a collagen hydrogel crosslinked at 37°C.
Centrifugation of hydrogel to create compartmentalized and layered models.

Main Results:

Successful generation of compartmentalized tumor-stroma models.
Encapsulation of a 96-well tumor mass within a 24-well stroma.
Increased collagen density and improved mechanical properties of hydrogels.

Conclusions:

The protocol provides a robust method for engineering advanced tumoroid models.
Enhanced mechanical properties improve the biomimicry of engineered tumor models.
This technique facilitates more accurate in vitro studies of tumor biology.