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Updated: May 21, 2025
09:04
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
20.8K
A wheat tandem kinase activates an NLR to trigger immunity
Renjie Chen1, Jian Chen2, Oliver R Powell1
1Plant Science Program, Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Science (New York, N.Y.)
|March 27, 2025
Summary
Wheat and barley gain disease resistance from a novel tandem kinase (TK) and NLR receptor complex. The AvrSr62 effector triggers this two-component system, revealing a new mechanism for plant immunity.
Area of Science:
- Plant molecular biology
- Plant-pathogen interactions
- Genetics and genomics
Background:
- Nucleotide-binding leucine-rich repeat (NLR) receptors are key to plant immunity.
- The function of tandem kinases (TKs) in disease resistance in wheat and barley is not well understood.
Purpose of the Study:
- To investigate the function of the SR62 locus in plant disease resistance.
- To elucidate the mechanism of the SR62 resistance complex involving tandem kinases and NLR receptors.
Main Methods:
- Gene cloning and identification of the SR62 locus.
- Effector protein identification and characterization (AvrSr62).
- Biochemical assays and structural modeling to analyze protein interactions.
Main Results:
- The SR62 locus is a digenic module encoding a tandem kinase (Sr62TK) and an NLR receptor (Sr62NLR).
- The AvrSr62 effector binds Sr62TK, displacing a second kinase domain and activating Sr62NLR.
- Overlapping binding sites on kinase domains mediate the activation cascade.
Conclusions:
- A novel two-component resistance complex involving tandem kinases and NLRs has been identified in cereals.
- Understanding this complex provides insights into plant immune signaling.
- This knowledge can aid in engineering durable disease resistance in crops.
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