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A novel photosensitive nanoprobe combined with CRISPR/Cas12a for dual signal amplification detection of ANGPTL2

  • 0Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer, Hospital of Nanjing Medical University, Nanjing, 210009, Jiangsu, China.
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March 27, 2025

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March 27, 2025

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Summary

This summary is machine-generated.

A new CRISPR/Cas biosensing platform detects angiopoietin-like protein 2 (ANGPTL2), a biomarker for colorectal cancer (CRC). This highly sensitive method shows promise for early CRC diagnosis using serum samples.

Area Of Science

  • Biotechnology
  • Molecular Diagnostics
  • Biomarker Discovery

Background

  • Early diagnosis of colorectal cancer (CRC) is crucial for patient outcomes.
  • Accurate quantification of low-abundance protein biomarkers in serum remains challenging.
  • Angiopoietin-like protein 2 (ANGPTL2) is a potential biomarker for CRC detection.

Purpose Of The Study

  • To develop a highly sensitive and specific biosensing platform for ANGPTL2 detection.
  • To integrate a CRISPR/Cas12 system with novel photosensitive nanoprobes for enhanced signal transduction.
  • To evaluate the potential of the developed biosensor for early CRC diagnosis in serum.

Main Methods

  • Development of a universal CRISPR/Cas biosensing platform (DPC-Cas).
  • Integration of photosensitive nanoprobes (DA/PL@Cu NPs) for signal amplification.
  • Utilizing the CRISPR/Cas12a system for specific detection and amplification of nucleic acid signals triggered by ANGPTL2.
  • Detection of ANGPTL2 in human serum samples.

Main Results

  • The DPC-Cas biosensor achieved ultrasensitive detection of ANGPTL2 down to 20.00 pg/mL.
  • The biosensor demonstrated high specificity and sensitivity in detecting ANGPTL2.
  • Successful detection of ANGPTL2 in serum samples was achieved, validating its clinical potential.
  • The platform integrates nanoprobes-assisted amplification with Cas12a self-amplification for robust signal generation.

Conclusions

  • The developed DPC-Cas biosensor offers a sensitive, specific, and user-friendly method for ANGPTL2 detection.
  • This platform shows significant potential for the early diagnosis of colorectal cancer.
  • The integration of photosensitive nanoprobes and CRISPR/Cas12 technology represents a promising advancement in biomarker detection.

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