Mg-Co double hydroxide/lactoferrin composite coating on Ti-based orthopedic implants: A pioneering strategy for...

In the field of orthopedic implants, peri-implant inflammation due to infection and poor osseointegration frequently causes metal implant failure. In this study, cobalt- and magnesium-containing double hydroxides (Co-Mg-Al-LDH) were first synthesized in situ on a titanium surface. Subsequently, amyloid lactoferrin (LF) was loaded into Co-Mg-Al-LDH to construct the LF/Co-Mg-TN composite coating. The coating was hydrophilic, corrosion-resistant, hemocompatible, biosafe, and had good mechanical qualities. LF/Co-Mg-TN showed some inhibitory efficacy against E. coli and S. aureus in vitro. RAW264.7 polarized to the M2-type as a result of LF/Co-Mg-TN upregulating the expression of anti-inflammatory genes and proteins and downregulating that of pro-inflammatory genes and proteins. In addition to enhancing ALP activity, collagen secretion, and cell mineralization in MC3T3-E1, LF/Co-Mg-TN also dramatically increased the expression of genes and proteins linked to osteogenesis and accelerated HUVEC motility, lumen-forming ability, and angiogenic growth factor expression. Significant antibacterial, anti-inflammatory, angiogenic, and osteogenic qualities were demonstrated by LF/Co-Mg-TN-coated titanium in vivo, which encouraged the regeneration of bone tissue. To sum up, the multifunctional amyloid/nanosheets coatings developed in this work offered a thorough therapeutic approach to fixing diseased bone deformities.