Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aptamer-based fluorescence "off-on" sensor for EGFR-positive lung cancer CTCs using streptavidin magnetic beads and N-CQDs@Ag⁺.

Mikrochimica acta·2026
Same author

HIF-1α enhances ferroptosis resistance in anaplastic thyroid carcinoma by suppressing ACSL4-mediated lipid metabolic homeostasis.

Cellular & molecular biology letters·2026
Same author

Reversible one-step acylation facilitates mitochondrial delivery of functional RNA.

Chemical science·2026
Same author

Cation homeostasis-related prognostic genes uncovered by transcriptomic analysis in breast cancer.

Translational cancer research·2026
Same author

Deciphering the Effect of Methyl 4‑Hydroxybenzoate on Breast Cancer by Bioinformatics and Experiments.

ACS omega·2026
Same author

Poria cocos-derived exosome-like nanoparticles ameliorate lymphedema by reprogramming fibroblast metabolism via enhanced TCA cycle flux.

Journal of nanobiotechnology·2026

Related Experiment Video

Updated: May 12, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

A site-specific fluorogenic probe for protein disulfide isomerase A1.

Yuli Zhuang1, Danqi Hong1, Wenjie Lang1

  • 1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.

Analytical Biochemistry
|March 27, 2025
PubMed
Summary

Researchers developed a novel fluorescent probe, LS, for sensitive and specific detection of Protein Disulfide Isomerase A1 (PDIA1) activity. This tool enables real-time monitoring of PDIA1 in health and disease.

Keywords:
Fluorogenic probePDIA1Specific labelingVinyl sulfone probe

More Related Videos

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
10:44

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
07:56

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Published on: May 30, 2021

Related Experiment Videos

Last Updated: May 12, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
10:44

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
07:56

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Published on: May 30, 2021

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Biology

Background:

  • Protein disulfide isomerase A1 (PDIA1) is crucial for protein folding and cellular homeostasis.
  • PDIA1 dysfunction is linked to various diseases, necessitating better tools for activity monitoring.
  • Existing methods for PDIA1 detection lack sensitivity and robustness.

Purpose of the Study:

  • To develop a sensitive and specific fluorescent probe for real-time monitoring of PDIA1 activity.
  • To investigate the potential of vinyl sulfone-based probes for studying PDIA1.
  • To create a tool for advancing research in PDIA1-associated diseases.

Main Methods:

  • Synthesis of vinyl sulfone-based fluorescent probes designed to target thiol groups.
  • Evaluation of probe performance, including fluorescence enhancement, specificity, and kinetics.
  • Characterization of probe binding to PDIA1, focusing on the active site cysteine residue.

Main Results:

  • The probe LS demonstrated an approximately 18-fold increase in fluorescence intensity upon binding to PDIA1.
  • LS exhibited high specificity for PDIA1 by targeting cysteine 397 in its active site.
  • Rapid fluorescence activation and stable spectral properties were observed for LS.

Conclusions:

  • The LS probe offers a powerful platform for dynamic, real-time monitoring of PDIA1 activity.
  • Its strong targeting, rapid response, and stability facilitate research into PDIA1's physiological and pathological roles.
  • This vinyl sulfone-based fluorescent probe advances the study of PDIA1 and related diseases.