Molecular imprinted photoresponse hybrid biocatalyst for ultrasensitive glutamate detection

A novel construction strategy for hybrid nanobiocatalyst was developed in this study by combining molecular imprinting technology with Fe₃O₄ nanoparticles hybrid MOF and enzyme immobilization technology. The impacts of molecular imprinting and the photothermal effect of Fe₃O₄ were investigated on the catalytic performance of the hybrid nanobiocatalyst. It was demonstrated that Fe₃O₄ nanoparticles exhibited a photothermal effect when exposed to multi-wavelength light. Leveraging the selective recognition ability of the molecular imprinting technique, the hybrid nanobiocatalyst showed a 15.8 % increase in catalytic activity for glutamate oxidase. Furthermore, under the influence of red light (650 nm), the photothermal effect induced by Fe₃O₄ accelerated the enzymatic reaction rate, resulting in a 23 % increase in enzyme activity. Benefiting from the specific recognition of the substrate by the Fe₃O₄ imprinted polymer, the hybrid nanobiocatalyst exhibited the rapid and highly sensitive detection of glutamate. Notably, this work provides an efficient strategy for constructing hybrid nanobiocatalyst with excellent properties.