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Propionate loading test for liver function during experimental liver necrosis in sheep.

Y Gröhn, M L Bruss, L A Lindberg

    American Journal of Veterinary Research
    |April 1, 1985
    PubMed
    Summary

    Liver necrosis in sheep impairs propionate to glucose conversion, indicated by elevated liver enzymes and prolonged propionate clearance. This suggests glucose levels can aid in diagnosing liver dysfunction in sheep.

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    Area of Science:

    • Veterinary Medicine
    • Animal Physiology
    • Biochemistry

    Background:

    • Propionate is a key gluconeogenic precursor in ruminants.
    • Liver function is crucial for glucose homeostasis.
    • Carbon tetrachloride (CCl4) is a common hepatotoxin used to induce liver damage in experimental models.

    Purpose of the Study:

    • To investigate the impact of experimentally induced liver necrosis on propionate gluconeogenesis in sheep.
    • To determine if glucose concentration can serve as a diagnostic marker for impaired liver function in sheep.
    • To assess the effect of liver damage on propionate clearance kinetics.

    Main Methods:

    • Sheep were administered CCl4 to induce liver necrosis.
    • Intravenous sodium propionate loads were administered before and after CCl4 treatment.

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  • Plasma liver-specific enzyme activities (iditol dehydrogenase, gamma-glutamyl-transferase) and sulfobromophthalein (BSP) half-life were measured.
  • Propionate clearance half-life (t1/2) was determined.

    • Main Results:

    • CCl4 administration induced liver necrosis and fatty infiltration.
    • Elevated plasma liver-specific enzyme activities and a significantly increased BSP half-life were observed post-CCl4 treatment.
    • The half-life of intravenous propionate load significantly increased in CCl4-treated sheep compared to controls.
    • Fasting alone did not cause significant changes in liver enzymes or propionate clearance.

    Conclusions:

    • Experimentally induced liver necrosis in sheep impairs the conversion of propionate to glucose.
    • Elevated liver enzymes and altered propionate metabolism kinetics are indicators of liver dysfunction in sheep.
    • Glucose concentration monitoring following a propionate load may aid in diagnosing disturbed liver function in sheep.