Related Experiment Video
Updated: May 3, 2026
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
Mitochondrial specific antioxidant MitoPBN mitigates oxidative stress and improves mitochondrial function in
Mahdieh Mehdipour1, Hossein Mohammadi2, Sahar Abdalkarim Salih3
1Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran. mehdipourmahdieh@yahoo.com.
Abstract:
Oxidative stress impairs sperm function and fertility in rams, most notably during cryopreservation, and is characterized by compromised sperm function and motility. Mitochondria contribute immensely towards sperm function, such as mitochondrial membrane potential (MMP) which is crucial for ATP production. The purpose of this study was to assess the role of MitoPBN supplementation on cryopreserved sperm quality in mature Ghezel ram sperm. Semen samples were treated with 0 (control), 100, 150, 200, and 250 µmol/L MitoPBN and then analyzed for key sperm factors. Results indicated that supplementation with 100 and 150 µmol/L MitoPBN significantly enhanced total motility, increasing to 52.36 ± 4.26% and 54.16 ± 3.19%, respectively. Progressive motility similarly improved to 24.82 ± 3.27% and 26.77 ± 3.46%, respectively. Additionally, sperm membrane integrity was markedly increased to 50.01 ± 4.22% and 52.54 ± 2.24%, while mitochondrial activity was significantly augmented from 35.14 ± 4.09% to 46.16 ± 4.02% and 50.26 ± 6.69%. Sperm viability also improved, rising to 48.99 ± 3.98% and 52.20 ± 3.17%, respectively. Notably, reactive oxygen species (ROS) levels were reduced to 2.95 ± 0.16% and 2.80 ± 0.11%, respectively, paralleled by enhancements in total antioxidant capacity (TAC; 1.85 ± 0.21% and 1.93 ± 0.16%, respectively) and glutathione peroxidase (GPx) activity (61.16 ± 4.77% and 63.36 ± 4.95%, respectively). Moreover, ATP content reached a peak of 116.29 ± 5.83 in the 150 µmol/L group. In conclusion, our results evidence that oxidative stress during ram sperm cryopreservation is effectively countered by MitoPBN, improving sperm quality.
Insights
MitoPBN supplementation significantly improves ram sperm quality after cryopreservation by reducing oxidative stress. This antioxidant treatment enhances sperm motility, viability, and mitochondrial function, crucial for fertility.
Area of Science:
- Reproductive biology and cryopreservation science.
- Oxidative stress and antioxidant research in animal reproduction.
Background:
- Oxidative stress negatively impacts ram sperm function and fertility, particularly during cryopreservation.
- Mitochondria are vital for sperm function, with mitochondrial membrane potential (MMP) essential for energy production.
Purpose of the Study:
- To evaluate the efficacy of MitoPBN supplementation in preserving Ghezel ram sperm quality post-cryopreservation.
- To investigate the protective effects of MitoPBN against oxidative stress-induced damage in ram spermatozoa.
Main Methods:
- Mature Ghezel ram semen samples were cryopreserved.
- Sperm samples were treated with varying concentrations of MitoPBN (0, 100, 150, 200, 250 µmol/L).
- Key sperm parameters including motility, membrane integrity, mitochondrial activity, viability, ROS levels, TAC, GPx activity, and ATP content were analyzed.
Main Results:
- MitoPBN supplementation at 100 and 150 µmol/L significantly enhanced total motility, progressive motility, sperm membrane integrity, mitochondrial activity, and viability.
- ROS levels were reduced, while total antioxidant capacity (TAC) and glutathione peroxidase (GPx) activity were increased by MitoPBN treatment.
- Optimal ATP content was observed at 150 µmol/L MitoPBN, indicating improved energy production.
Conclusions:
- MitoPBN effectively mitigates oxidative stress during ram sperm cryopreservation.
- Supplementation with MitoPBN demonstrably improves cryopreserved ram sperm quality, offering a promising strategy for fertility preservation.
More Related Videos
08:04Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
Published on: August 16, 2021
08:32Author Spotlight: Advancing Male Infertility Research by Unraveling Sperm Metabolism and Mitochondrial Function
Published on: June 23, 2023
