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A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
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Isolation of vanA-Mediated Vancomycin-Resistant Enterococcus faecalis (ST1912/CC116) and Enterococcus faecium

Sangjukta Roy1, Meiji Soe Aung2, Shyamal Kumar Paul3

  • 1Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, Bangladesh.

Antibiotics (Basel, Switzerland)
|March 28, 2025
PubMed
Abstract

Insights

Antimicrobial resistance in Enterococcus species, a major nosocomial pathogen, was studied in Bangladesh. Vancomycin resistance (vanA) and linezolid resistance (optrA-fexA) were identified, highlighting potential public health threats.

Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Antimicrobial resistance

Background:

  • Enterococcus species are significant causes of hospital-acquired infections globally.
  • Understanding the current antimicrobial resistance (AMR) landscape of Enterococcus in Bangladesh is crucial for effective treatment and control.

Purpose of the Study:

  • To analyze antimicrobial resistance determinants and virulence factors in Enterococcus isolates from infectious diseases in Bangladesh.
  • To identify the prevalence of specific resistance genes and sequence types (STs) in clinical Enterococcus isolates.

Main Methods:

  • Clinical isolates of Enterococcus were collected from a tertiary care hospital in Bangladesh.
  • Antimicrobial susceptibility testing was performed using broth microdilution.
  • Resistance genes and virulence factors were detected using PCR and sequencing; multilocus sequence typing (MLST) was employed for E. faecalis and E. faecium.

Main Results:

  • A total of 143 Enterococcus isolates were analyzed, predominantly E. faecalis (n=135).
  • High resistance rates were observed for erythromycin (87%) and levofloxacin (62%) in E. faecalis. High-level gentamicin resistance was prevalent in both E. faecalis (30%) and E. faecium (86%).
  • Vancomycin resistance (vanA) was found in one E. faecalis and one E. faecium isolate. Linezolid resistance (optrA-fexA) was detected in three E. faecalis isolates belonging to different STs.

Conclusions:

  • This study reports the presence of vancomycin-resistant Enterococcus (VRE) harboring vanA in human clinical isolates in Bangladesh.
  • The findings indicate a potential for the spread of optrA-mediated linezolid resistance across multiple E. faecalis lineages in the region.
Keywords:
Enterococcus faecalisEnterococcus faeciumSTVREantimicrobial resistanceoptrAvanA

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