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Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In

Hana Yao¹, Hiromitsu Toyoda¹, Naoki Takada¹

¹Department of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, Japan.

|March 28, 2025

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Plasma-activated medium (PAM) effectively reduced synovial sarcoma growth in preclinical models. This novel treatment induced cell apoptosis via reactive oxygen species (ROS) accumulation, showing promise as a new therapeutic option.

Area of Science:

Biomedical Engineering
Oncology
Cell Biology

Background:

Plasma-activated medium (PAM) exhibits anti-tumor properties against various cancers.
The efficacy of PAM against synovial sarcoma and its mechanisms remain largely unexplored.
Synovial sarcoma presents significant treatment challenges, necessitating novel therapeutic strategies.

Purpose of the Study:

To investigate the anti-tumor effects of PAM on synovial sarcoma.
To elucidate the underlying mechanisms, focusing on reactive oxygen species (ROS) and apoptosis.
To evaluate the safety and efficacy of PAM in a preclinical synovial sarcoma model.

Main Methods:

Utilized the human synovial sarcoma cell line HS-SY-II to assess cell viability post-PAM treatment.

Keywords:

anti-tumor effect cell apoptosis intracellular reactive oxygen species (ROS)non-thermal atmospheric pressure plasma plasma-activated medium (PAM)synovial sarcoma

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Administered local PAM injections in a synovial sarcoma xenograft murine model to evaluate tumor growth and side effects.

Quantified intracellular ROS levels and apoptosis induction by PAM, using a ROS scavenger to confirm mechanism involvement.

Main Results:

PAM significantly reduced synovial sarcoma cell viability in vitro.
Local PAM injection suppressed tumor volume and weight in vivo without observable side effects.
PAM induced significant apoptosis and intracellular ROS accumulation, which were crucial for its anti-tumor effect.

Conclusions:

PAM demonstrates significant in vitro and in vivo anti-tumor efficacy against synovial sarcoma.
The anti-cancer mechanism involves PAM-induced intracellular ROS accumulation leading to apoptosis.
PAM represents a promising, safe, and novel therapeutic option for synovial sarcoma treatment.