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Updated: Jun 16, 2026
![Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60786.jpg&w=3840&q=50)
Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions
Published on: July 17, 2020
Robustness of the Cupriavidus necator-Catalyzed Production of α-Humulene
Lucas Becker1, Emely Dietz1, Dirk Holtmann2
1Bioprocess Intensification, Institute of Bioprocess Engineering and Pharmaceutical Technology, Technische Hochschule Mittelhessen, 35390 Giessen, Germany.
Abstract:
The increasing global demand for natural substances such as the sesquiterpene α-humulene makes optimizing microbial production essential. A production process using the versatile host Cupriavidus necator has been recently improved by adjusting the minimal media and process parameters. Understanding microbial and process robustness is key to ensuring consistent performance under different conditions. This study is the first to investigate and quantify the robustness of microbial α-humulene production and biomass formation using C. necator pKR-hum. Established process improvements and the impact of common or individual precultures were analyzed and quantified for their effect on the robustness of product and biomass formation. We report a robust α-humulene production process with even more consistent biomass formation using C. necator pKR-hum. Even with a simulated process disturbance, 79% of the maximum α-humulene level was still produced. Overall, our results show that the α-humulene production process using C. necator pKR-hum is highly robust, demonstrating its resilience to process disturbances and suitability for further industrial applications.
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