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Published on: February 28, 2020
A positive feedback loop between FOSB and miR-133b controls colon cancer cell proliferation
Wanwan Li1,2, Qionggui Hu1, Changwei Lin1
1Department of Gastrointestinal Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China.
Acta Biochimica Et Biophysica Sinica
|March 28, 2025
Summary
FOSB acts as a tumor suppressor in colon cancer (CC). It inhibits CC cell proliferation and promotes apoptosis through a FOSB-miR-133b-POU2F1 feedback loop, suggesting therapeutic potential.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- FOSB, a FOS gene family member, has varied roles in tumors, but its function in colon cancer (CC) is unclear.
- Understanding FOSB's role is crucial for developing targeted CC therapies.
Purpose of the Study:
- To investigate the role and mechanism of FOSB in colon cancer.
- To analyze the relationship between FOSB expression and clinical parameters in CC patients.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC) for FOSB expression.
- In vitro cell proliferation, cell cycle, and apoptosis assays.
- In vivo xenograft models and chromatin immunoprecipitation (ChIP) assays.
Main Results:
- FOSB expression was downregulated in CC tissues compared to normal tissues.
- FOSB overexpression inhibited CC cell proliferation and induced apoptosis.
- A feedback loop involving FOSB, miR-133b, and POU2F1 was identified, suppressing CC growth.
Conclusions:
- FOSB functions as a tumor suppressor gene in colon cancer.
- The FOSB-miR-133b-POU2F1 feedback loop is a key mechanism inhibiting CC proliferation.
- FOSB represents a potential therapeutic target for colon cancer treatment.
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