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Updated: May 5, 2026

Identifying Coronary Artery Calcification on Non-gated Computed Tomography Scans
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Identifying Coronary Artery Calcification on Non-gated Computed Tomography Scans

Published on: August 28, 2018

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Diagnostic performance of RAC2 combined with CT-FFR parameters in coronary heart disease

Zhanying Zhu1, Jiani Xu1, Haitao Wang1

  • 1Department of Radiology, No. 903 Hospital of PLA Joint Logistic Support Force, Hangzhou, China.

Minerva Cardiology and Angiology
|March 28, 2025
PubMed

Insights

RAC2, a key gene in coronary heart disease (CHD), promotes inflammation and predicts poor prognosis. Combining RAC2 with CT fractional flow reserve (CT-FFR) significantly improves CHD diagnosis accuracy.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Coronary heart disease (CHD) poses a significant health burden, particularly in the elderly.
  • CT fractional flow reserve (CT-FFR) shows promise for diagnosing cardiovascular diseases.
  • Identifying novel biomarkers is crucial for enhancing CHD diagnostic accuracy.

Purpose of the Study:

  • To identify key genes associated with CHD from the GEO database.
  • To investigate the role of RAC2 in CHD pathogenesis and its potential as a diagnostic biomarker.
  • To evaluate the combined diagnostic value of RAC2 and CT-FFR for CHD.

Main Methods:

  • Gene screening using the GEO database.
  • ROC curve analysis and logistic regression using GraphPad and SPSS software.
  • In vitro inflammatory cell model using TNF-α stimulated HMVEC-Cs to assess RAC2 function.

Main Results:

  • RAC2 was found to be highly expressed in CHD patients, with expression levels decreasing after nitric ester therapy.
  • The combination of RAC2 and CT-FFR demonstrated superior diagnostic value for CHD (AUC=0.971).
  • RAC2 was identified as an independent risk factor for poor prognosis in CHD patients receiving nitric ester drugs (AUC=0.888).
  • RAC2 modulated the expression of inflammatory markers (NF-κB, NLRP3, IL-1β, IL-6) in TNF-α-induced HMVEC-Cs.

Conclusions:

  • RAC2 promotes inflammatory responses in cardiac microvascular endothelial cells.
  • RAC2 serves as a predictor of poor prognosis in CHD patients.
  • The combined assessment of RAC2 and CT-FFR offers a robust diagnostic strategy for CHD.
Abstract

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