The landscape of long non-coding RNA during cSCC progression
- Max Bone 1,2, Daniel Schreyer 1,2, Mairi Treanor-Taylor 1,2, Charlotte M Proby 3, Catherine A Harwood 4,5, Irene M Leigh 4, Peter Bailey 1,6, Gareth J Inman 1,2
- Max Bone 1,2, Daniel Schreyer 1,2, Mairi Treanor-Taylor 1,2
- 1School of Cancer Sciences, University of Glasgow, UK.
- 2Cancer Research UK Scotland Institute, Glasgow, UK.
- 3Molecular and Clinical Medicine, School of Medicine, University of Dundee, Dundee, UK.
- 4Centre for Cell Biology and Cutaneous Research, Blizzard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
- 5Department of Dermatology, The Royal London Hospital, Barts Health NHS Trust, London, UK.
- 6Botton-Champalimaud Pancreatic Cancer Centre, Avenida Brasilia, Lisboa, Portugal.
- 0School of Cancer Sciences, University of Glasgow, UK.
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View abstract on PubMed
Summary
This summary is machine-generated.Long non-coding RNAs (lncRNAs) are key drivers in cutaneous squamous cell carcinoma (cSCC) progression. This study identified lncRNA signatures that predict poor prognosis and revealed LINC00941
Area Of Science
- Oncology
- Molecular Biology
- Genomics
Background
- Cutaneous squamous cell carcinoma (cSCC) is a prevalent cancer with significant morbidity, especially in its metastatic form.
- The progression from pre-malignant Actinic Keratosis (AK) to invasive and metastatic cSCC involves poorly defined molecular mediators.
- Long non-coding RNAs (lncRNAs) are emerging as critical, yet understudied, players in cSCC pathogenesis.
Purpose Of The Study
- To comprehensively map the landscape of lncRNA expression across the cSCC disease continuum.
- To identify functional lncRNA drivers implicated in cSCC progression.
- To develop and validate a prognostic lncRNA signature.
Main Methods
- Analysis of bulk RNA sequencing data from 110 patient samples spanning normal skin, AK, primary cSCC, and metastases.
- Development of a bioinformatics pipeline for lncRNA function inference via co-expression patterns.
- In vitro knockdown experiments to investigate the functional role of LINC00941.
Main Results
- lncRNA expression profiles accurately distinguish disease states and progression along the cSCC continuum.
- A 267-lncRNA signature was developed, correlating with a progenitor-like state and predicting poor prognosis in head and neck squamous cell carcinoma (HNSC) and pancreatic adenocarcinoma (PAAD).
- Knockdown of LINC00941 demonstrated its necessity for cell proliferation and colony formation in vitro.
Conclusions
- This study provides a detailed transcriptomic map of lncRNAs in cSCC, highlighting their functional significance.
- lncRNAs serve as potential biomarkers and drivers of disease progression in cSCC.
- The findings suggest lncRNAs may also play roles in other cancer types.
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