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Updated: Jul 9, 2026

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
Engineering of Glycosyltransferase for Efficient Biosynthesis of Salidroside
Fucheng Zhu1, Zixu Yan1,2, Kexue Zhao1,2
1College of Biological and Pharmaceutical Engineering, Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Anhui Province Key Laboratory for Quality Evaluation and Improvement of Traditional Chinese Medicine, West Anhui University, Lu'an 237012, China.
Abstract:
Salidroside has been widely used in the cosmetic and medicinal industries. Previously, potential glycosyltransferase UGTBS was obtained for salidroside synthesis. However, the catalytic efficiency for salidroside was undesirable. In this study, a semirational design was applied to engineer UGTBS. The quadruple mutant M4 (I62N/S129T/F168W/Y316S) showed significantly enhanced salidroside synthesis. A conversion rate of 94.7% was obtained using mutant M4, which was 2.2-fold higher than that of the wild-type. The regioselectivity of mutant M4 toward tyrosol hydroxyl was also improved, resulting in 97.9% salidroside in the total product, which was 1.2-fold higher than that of the wild type. Kinetic constants and molecular simulations indicated that increased affinity and altered conformation of the binding pocket accounted for the enhanced salidroside synthesis. Furthermore, a fed-batch cascade reaction strategy was used, and over 183 mM salidroside was obtained. The engineered mutant M4 demonstrated precise catalysis for salidroside formation.
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