Multifunctional nanozymatic biosensors: Awareness, regulation and pathogenic bacteria detection

It is estimated that approximately 700,000 fatalities occur annually due to infections attributed to various pathogens, which are capable of dissemination via multiple environmental vectors, including air, water, and soil. Consequently, there is an urgent need to enhance and refine rapid detection technologies for pathogens to prevent and control the spread of associated diseases. This review focuses on applying nanozymes in constructing biosensors, particularly their advancement in detecting pathogenic bacteria. Nanozymes, which are nanomaterials exhibiting enzyme-like activity, combine unique magnetic, optical, and electronic properties with structural diversity. This blend of characteristics makes them highly appealing for use in biocatalytic applications. Moreover, their nanoscale dimensions facilitate effective contact with pathogenic bacteria, leading to efficient detection and antibacterial effects. This article briefly summarizes the development, classification, and strategies for regulating the catalytic activity of nanozymes. It primarily focuses on recent advancements in constructing biosensors that utilize nanozymes as probes for sensitively detecting pathogenic bacteria. The discussion covers the development of various optical and electrochemical biosensors, including colorimetric, fluorescence, surface-enhanced Raman scattering (SERS), and electrochemical methods. These approaches provide a reliable solution for the sensitive detection of pathogenic bacteria. Finally, the challenges and future development directions of nanozymes in pathogen detection are discussed.