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Dengue virus induced polypeptide synthesis. Brief report.

S Ozden, B Poirier

    Archives of Virology
    |January 1, 1985
    PubMed
    Summary

    Researchers studied Dengue 2 virus polypeptide synthesis in BHK21 cells, identifying nine to ten viral polypeptides, including three glycoproteins. Evidence suggests restricted proteolytic cleavage may occur during processing of these viral proteins.

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    ARE THYROID NODULES AN OBSTACLE TO MINIMAL INVASIVE PARATHYROID SURGERY? A SINGLE-CENTER STUDY FROM AN ENDEMIC GOITER REGION.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Dengue virus is a significant global health concern.
    • Understanding viral protein synthesis is crucial for developing antiviral strategies.
    • BHK21 cells are a common model system for studying flaviviruses like Dengue 2.

    Purpose of the Study:

    • To investigate the synthesis and processing of Dengue 2 virus-induced polypeptides in infected BHK21 cells.
    • To identify viral glycoproteins and determine if large polypeptide processing occurs.

    Main Methods:

    • Infection of BHK21 cells with Dengue 2 virus.
    • Identification of viral polypeptides using techniques like tunicamycin treatment.
    • Pulse-chase experiments to study protein processing.
    • Use of amino-acid analogs, protease inhibitors, and pactamycin.
    • Immunoprecipitation with anti-dengue 2 serum.

    Main Results:

    • Nine to ten virus-induced polypeptides were identified.
    • Three of these polypeptides were confirmed as glycoproteins.
    • Evidence for the processing of a large polypeptide (P130) was observed.
    • Label transfer between smaller polypeptides suggested restricted proteolytic cleavage.

    Conclusions:

    • Dengue 2 virus infection leads to the synthesis of multiple viral polypeptides, including glycoproteins.
    • The study provides evidence supporting the processing of large viral polypeptides, potentially through restricted proteolytic cleavage.

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