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Related Experiment Video

Updated: May 20, 2025

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
08:02

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Published on: April 25, 2016

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Collecting equine whole blood in glass tubes does not decrease adrenocorticotropic hormone concentration compared to

Emma D Stapley, François-René Bertin

    Journal of the American Veterinary Medical Association
    |March 28, 2025
    PubMed
    Summary
    This summary is machine-generated.

    Glass or plastic EDTA tubes are suitable for collecting equine blood for ACTH measurement. Storing whole blood for up to six hours at 4°C did not affect results, ensuring accurate pituitary pars intermedia dysfunction diagnosis.

    Keywords:
    diagnosisendocrinologyhypertrichosislaminitispituitary pars intermedia dysfunction

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    Area of Science:

    • Veterinary Endocrinology
    • Equine Internal Medicine
    • Diagnostic Assay Development

    Background:

    • Pituitary pars intermedia dysfunction (PPID) diagnosis relies on measuring adrenocorticotropic hormone (ACTH).
    • Concerns exist regarding ACTH binding to glass blood collection tubes, potentially affecting measurement accuracy.
    • The clinical impact of using glass versus plastic EDTA tubes for equine ACTH sample collection is unclear.

    Purpose of the Study:

    • To evaluate the effect of glass versus plastic EDTA tubes on equine ACTH concentrations.
    • To determine the impact of storage duration (0-6 hours) on ACTH stability in collected blood samples.
    • To assess the clinical relevance of tube material for ACTH measurement in horses.

    Main Methods:

    • A cohort study involving 9 horses (>12 years old) with and without PPID.
    • Whole blood was collected in both glass and plastic EDTA tubes.
    • Samples were stored at 4°C for 0, 2, 4, or 6 hours before centrifugation and immediate ACTH measurement via chemiluminescent assay.

    Main Results:

    • No significant difference in plasma ACTH concentrations was observed between glass and plastic EDTA tubes.
    • Storage time up to 6 hours at 4°C did not significantly alter ACTH levels.
    • Tube type and storage duration did not significantly impact measured ACTH concentrations.

    Conclusions:

    • Equine whole blood collected in glass EDTA tubes can be stored for up to 6 hours at 4°C without significantly affecting ACTH concentrations compared to plastic tubes.
    • Both glass and plastic EDTA tubes are suitable for the collection and same-day analysis of equine ACTH.
    • These findings support the use of either tube type for reliable ACTH measurement in diagnosing equine pituitary pars intermedia dysfunction.